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arp 11346 pvsv g addgene  (Addgene inc)


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    Structured Review

    Addgene inc arp 11346 pvsv g addgene
    Arp 11346 Pvsv G Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+2gtwy/pACT2%2E2gtwy+(Plasmid+%2311346)/pm37127492-331-65-67
    Average 92 stars, based on 7 article reviews
    arp 11346 pvsv g addgene - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Two Hybrid Assay:

    Article Title: Fission Yeast Mto1 Regulates Diversity of Cytoplasmic Microtubule Organizing Centers
    Article Snippet: .. For two-hybrid assay of Mto1-Myp2 interaction, a Myp2 cDNA [57] was subcloned into pACT2.2gtwy (Addgene plasmid 11346, deposited by G. Caldwell). ..

    Plasmid Preparation:

    Article Title: Fission Yeast Mto1 Regulates Diversity of Cytoplasmic Microtubule Organizing Centers
    Article Snippet: .. For two-hybrid assay of Mto1-Myp2 interaction, a Myp2 cDNA [57] was subcloned into pACT2.2gtwy (Addgene plasmid 11346, deposited by G. Caldwell). ..

    Article Title: SUMO-Targeted Ubiquitin Ligases (STUbLs) Reduce the Toxicity and Abnormal Transcriptional Activity Associated With a Mutant, Aggregation-Prone Fragment of Huntingtin
    Article Snippet: .. For Htt localization and auto-activation assays, Htt with 25Q, 55Q, and 97Q were PCR-amplified using NEB Q5 hot start high-fidelity polymerase 2× master mix (Cat # M0494S) and cloned into the pCR8/GW/TOPO entry vector (Life Technologies) and then recombined into either pAG414GAL-ccdB-DsRed (Addgene #14359) forming GAL-97QHtt-DsRed/TRP1/CEN (BOK 1213) or pACT2.2gtwy (Addgene # 11346) forming ADH1-GAL4AD-25QHtt/LEU2/2μ (BOK 1207), ADH1-GAL4AD-55QHtt/LEU2/2μ (BOK1209) and ADH1-GAL4AD-97QHtt/LEU2/2μ (BOK 1215). ..

    Article Title: Arabidopsis Membrane-anchored Ubiquitin-fold (MUB) Proteins Localize a Specific Subset of Ubiquitin-conjugating (E2) Enzymes to the Plasma Membrane
    Article Snippet: .. Construction of plasmids pDEST-GBKT7 ( 33 ); pACT2.2gtwy (Addgene plasmid 11346); pEarleyGate (pEG) 100, pEG104, pEG201, and pEG202 ( 34 ); pGGWA and pHGWA ( 35 ); pET28a-UbcH5c and pET28a-UbcH5c S22R (Addgene plasmids 12643 and 12644) ( 12 ); and pET28b- AtMUB1–6 ( 26 ) was described previously. ..

    Article Title: Arabidopsis Membrane-anchored Ubiquitin-fold (MUB) Proteins Localize a Specific Subset of Ubiquitin-conjugating (E2) Enzymes to the Plasma Membrane
    Article Snippet: .. Construction of plasmids pDEST-GBKT7 (33); pACT2.2gtwy (Addgene plasmid 11346); pEarleyGate (pEG) 100, pEG104, pEG201, and pEG202 (34); pGGWA and pHGWA (35); pET28a-UbcH5c and pET28a-UbcH5c S22R (Addgene plasmids 12643 and 12644) (12); and pET28b-AtMUB1–6 (26) was described previously. ..

    Polymerase Chain Reaction:

    Article Title: SUMO-Targeted Ubiquitin Ligases (STUbLs) Reduce the Toxicity and Abnormal Transcriptional Activity Associated With a Mutant, Aggregation-Prone Fragment of Huntingtin
    Article Snippet: .. For Htt localization and auto-activation assays, Htt with 25Q, 55Q, and 97Q were PCR-amplified using NEB Q5 hot start high-fidelity polymerase 2× master mix (Cat # M0494S) and cloned into the pCR8/GW/TOPO entry vector (Life Technologies) and then recombined into either pAG414GAL-ccdB-DsRed (Addgene #14359) forming GAL-97QHtt-DsRed/TRP1/CEN (BOK 1213) or pACT2.2gtwy (Addgene # 11346) forming ADH1-GAL4AD-25QHtt/LEU2/2μ (BOK 1207), ADH1-GAL4AD-55QHtt/LEU2/2μ (BOK1209) and ADH1-GAL4AD-97QHtt/LEU2/2μ (BOK 1215). ..

    Clone Assay:

    Article Title: SUMO-Targeted Ubiquitin Ligases (STUbLs) Reduce the Toxicity and Abnormal Transcriptional Activity Associated With a Mutant, Aggregation-Prone Fragment of Huntingtin
    Article Snippet: .. For Htt localization and auto-activation assays, Htt with 25Q, 55Q, and 97Q were PCR-amplified using NEB Q5 hot start high-fidelity polymerase 2× master mix (Cat # M0494S) and cloned into the pCR8/GW/TOPO entry vector (Life Technologies) and then recombined into either pAG414GAL-ccdB-DsRed (Addgene #14359) forming GAL-97QHtt-DsRed/TRP1/CEN (BOK 1213) or pACT2.2gtwy (Addgene # 11346) forming ADH1-GAL4AD-25QHtt/LEU2/2μ (BOK 1207), ADH1-GAL4AD-55QHtt/LEU2/2μ (BOK1209) and ADH1-GAL4AD-97QHtt/LEU2/2μ (BOK 1215). ..



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